Heparinase II cleaves heparin and heparan sulfate. It is mainly used for quality control of heparin products.
Synonyms: Heparitinase IIGene Source: Native flavobacterium heparinumEC Number: Not assignedCAS Number: 149371-12-0MDL Number: MFCD00131319Catalyzed Reaction: The enzyme cleaves, via an elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and uronic acid residues (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin and heparan sulfate, with the heparan sulfate activity being about twice as high as the heparin activity.Substrate Specificity: Heparin; heparan sulfate.
Product Specification / Models
Molecular weight: 84.1 kDaIsoelectric point: 9.1 - 9.2pH optimum for activity: 7.6pH range for activity: 5 - 9Optimal temperature range: 20 degree - 37 degreePurity: NLT 98 % by reversed phase HPLC analysis.Specific Activity: 100 IU/mg, 1 IU/250 uL. One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30 degree and pH 7.5.Stability: expiration is 12 months from manufacturing date, frozen at -20 degree in PBS.
Application / Models
As research reagent (glycosaminoglycan degradation).For the preparation of di- and oligo-saccharides of heparin and heparan sulfate and the preparation of oligosaccharide libraries.
Catalog number: AG00-6512Packing size: 1 IUPrice: 1200USD
We are Adhoc International in Beijing, China, focusing on heparin detection reagents, such as heparinases, chromogenic substrates and heparin dissacharides.