Heparinase II

Origin
China
Minimum Order
1 IU
Inspection
N/A
Packing
Stored in vials with dry ice to keep it cool
Delivery
4 weeks
Payment
TT 100% in advance
Product Description
Heparinase II cleaves heparin and heparan sulfate. It is mainly used for quality control of heparin products.
Product Feature
Synonyms: Heparitinase IIGene Source: Native flavobacterium heparinumEC Number: Not assignedCAS Number: 149371-12-0MDL Number: MFCD00131319Catalyzed Reaction: The enzyme cleaves, via an elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and uronic acid residues (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin and heparan sulfate, with the heparan sulfate activity being about twice as high as the heparin activity.Substrate Specificity: Heparin; heparan sulfate.
Product Specification / Models
Molecular weight: 84.1 kDaIsoelectric point: 9.1 - 9.2pH optimum for activity: 7.6pH range for activity: 5 - 9Optimal temperature range: 20 degree - 37 degreePurity: NLT 98 % by reversed phase HPLC analysis.Specific Activity: 100 IU/mg, 1 IU/250 uL. One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30 degree and pH 7.5.Stability: expiration is 12 months from manufacturing date, frozen at -20 degree in PBS.
Application / Models
As research reagent (glycosaminoglycan degradation).For the preparation of di- and oligo-saccharides of heparin and heparan sulfate and the preparation of oligosaccharide libraries.
Other Information
Catalog number: AG00-6512Packing size: 1 IUPrice: 1200USD
We are Adhoc International in Beijing, China, focusing on heparin detection reagents, such as heparinases, chromogenic substrates and heparin dissacharides.

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Adhoc International Technologies Co., Ltd

China China
Contact Person: Frank Li
Telephone: 86-10-57114681
Location: Beijing, China
Fax:86-10-64321632
Business Type:Manufacturer, Exporter
Main Target Region:World Wide
Address:
No. 63, Maquanying Fourth Zone,Chaoyang District
Main Product:
Heparinases/detection reagents/heparin dissacharides
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