This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Mouse Angiopoietin 1 (ANG-1). Add Angiopoietin 1(ANG-1)to the wells, which are pre-coated with Angiopoietin 1(ANG-1)monoclonal antibody and then incubate. After that, add anti ANG-1 antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Mouse Angiopoietin 1 (ANG-1) are positively correlated.
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